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若配制500m1PDA斜面培养基,其配方和配制过程如何

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正确答案:

配方是:马铃薯100g,糖10g,琼脂10~12g,水500ml。

配制过程是:

⑴按配方备料、称量

⑵煮制马铃薯洗净,去皮及芽眼,切碎,加占配制量1.3倍的水,煮沸20min。用纱布过滤,取500ml滤液。

⑶分装通过漏斗装置分装于试管中。装量约占试管高度的1/4,勿玷污管口及管壁。7~10支试管扎一捆,棉塞部分用牛皮纸包严。

⑷灭菌在加热排冷气后,压力在0.1~0.15MPa灭菌30min。降压至零时取出培养基趁热摆成斜面。

答案解析:

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